Temperature Control Modes

The instrument uses one of two temperature control modes to determine when the sample reaches the target temperature in a protocol:

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Block mode — when the sample volume is set to 0 μl, the instrument assumes the sample temperature is the same as the measured block temperature and automatically sets this mode.

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Calculated mode — when the sample volume is set to more than 0 µl (non-zero volume appropriate for the block), the instrument calculates the sample temperature based on the sample volume. This is the standard mode.

Important: When the protocol is started on an instrument, reaction volume range may be restricted to a narrower range, depending on the instrument.