Editing the Plate

When you click Continue to Plate Setup, the plate view opens. All wells display the default configuration.

Note: BR.io accepts standard and deepwell plates.

Your plate setup instructs BR.io how to analyze and display the information collected by the instrument in the completed run file. The main elements are identified in the following graphic, and described in the associated legend.

Note: The run setup order appears in the bottom navigation bar, with the active process highlighted.

Legend 

File name.

A default name is assigned initially; you can rename the file in the Run Details page.

Quick Save option, which allows you to save as you configure the plate.

Clear option, which allows you to clear the contents of selected wells.

The option is enabled when you select a well.

Plate layout section; selected wells are highlighted with a blue border.

The Plate Layout contains Target Tags and Sample Tags (described below), which convey the contents of each well:

Sample Tags

Rectangular tags at the top of each well that convey information about the sample analyzed in the well.

The Sample Type and Replicate ID (if defined) are displayed on the first row of the tag.
The Sample Name (if defined) is displayed on the 2nd row of the tag for all sample types except Standard.
For a Standard, the concentration is displayed in place of the Sample Name. Sample tags are color-coded by Sample Type.

 

Target Tags

Rounded rectangular tags below the Sample Tag in each well convey information about the target and fluorophore detected in the well.

A Target Tag is present for each Target/Fluorophore assigned to the well:

If the Target Name is defined, it is displayed in a Target Tag. Target tags are color-coded.
If the Target Name is undefined, the Fluorophore is displayed instead of the Target Name, and the color matches the color assigned to the Fluorophore.

 

Panel for entering plate information, which changes based on the setup step:

The first panel display is for selecting the plate details, including scan mode, plate size, and plate type. You can also click the Copy plate from another run link to select plate details from a previous run that matches the run you are configuring.
The second panel display is for defining targets, fluorophores, and sample.\

Clicking the button moves to the next setup step.

Note the following:

You can edit the plate before, during, or after the run.
If you have navigated to another page, use the Plate link in the CFX workflow navigator to return to the plate editor. For information, see Using the CFX Workflow Navigator.
You are not required to create or edit a plate layout before executing the run on the instrument. To defer the full plate configuration, ensure that you have properly set up the Plate Details and then skip to Specifying the Run Details.